<b>Integrating Single-Cell RNA Sequencing and Spatial Transcriptomics Reveals the Role of

Single-cell RNA sequencing and spatial transcriptomics data from mouse syngeneic and allogeneic heterotopic tracheal transplantation models investigating bronchiolitis obliterans. The study, authored by Dongfang Qiao and last updated in April 2026, identifies a recipient-derived Thy-1-positive macrophage population undergoing macrophage-to-myofibroblast transition. Data likely includes gene expression profiles and spatial coordinates for cell populations involved in airway fibrosis.

Use Cases

• Analyze macrophage-to-myofibroblast transition trajectories based on Thy-1 expression dynamics mentioned in the description
• Investigate spatial distribution patterns of fibrotic cell subsets based on extracellular matrix remodeling signatures
• Model the regulatory role of TGF-β and IL-4/IL-13 in fibrotic niche specialization
• Validate Thy-1 as a potential therapeutic target for mitigating airway fibrosis using in vitro correlates

Strengths

• Integrates single-cell RNA sequencing with spatial transcriptomics for multimodal analysis
• Focuses on a novel cellular source (Thy-1+ macrophages) in a clinically significant pathology (bronchiolitis obliterans)
• Includes in vitro experimental validation of key mechanistic findings

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Data is derived from mouse models; direct applicability to human pathology may require validation

Provenance

Source

figshare, author Dongfang Qiao

Collection Method

Generated from mouse transplantation models combined with histopathology, ELISA, single-cell RNA sequencing, and spatial transcriptomics.

Time Range

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Freshness

Last updated 2026-04 09:16:24; freshness should be verified

Geography

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