JANAS: Cryo-EM Datasets for Protein Complexes and RNA Structures

Mauro Maiorca deposited data associated with the manuscript 'Resolving Functional States in Cryo-EM Datasets with JANAS'. The archive includes datasets for the JANAS pipeline applied to several protein and RNA complexes, including the 5-HT1BR–Go receptor, 46Q HTT–HAP40, O. iheyensis group II intron, and OLE RNA homodimer. Datasets include refined 3D maps, atomic models, and related metadata.

Use Cases

• Benchmarking particle selection algorithms based on the Structural Cross-correlation Index (SCI)
• Evaluating class reassignment reproducibility using independently repicked particle stacks
• Analyzing conformational states within ligand-binding pockets from focused classification
• Training or validating models for RNA-only structure determination
• Comparing method performance using the provided SCI_test archive source data

Strengths

• Includes data from multiple publicly deposited EMPIAR entries (10308, 10667, 12707)
• Provides specific particle counts for ligand-binding pocket classes (16,736 and 15,349 particles)
• Contains refined 3D maps, atomic models, and related metadata for each dataset

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• Raw particle stacks from referenced EMPIAR entries are not included, requiring separate download

Provenance

Source

figshare

Collection Method

Associated with the application of the JANAS cryo-EM data processing pipeline.

Freshness

Last updated 2026-05-31 12:18:51; freshness should be verified