Metagenome-Assembled Genomes from a Sulfur-Rich Karst Aquifer in Brazil

Northeastern Brazil's Irecê Basin provides the location for this collection of medium- and high-quality metagenome-assembled genomes (MAGs) from groundwater microbial communities. The dataset was created by author Renato Romano from shotgun metagenomic sequencing of samples under contrasting hydrological and redox conditions, with raw reads deposited in NCBI SRA under BioProject PRJNA1463332. It was last updated on May 9, 2026.

Use Cases

• Study sulfur-cycling microbial communities based on the described focus on sulfur-rich aquifer environments.
• Analyze genome quality and contamination metrics based on the application of MIMAG standards and CheckM tools.
• Compare microbial community structure under different redox conditions based on the sampling of contrasting hydrological states.
• Benchmark metagenomic assembly and binning pipelines based on the described use of MEGAHIT, CONCOCT, and anvi'o.

Strengths

• Includes only medium- and high-quality genomes, defined as having ≥50% completeness and ≤10% contamination.
• Genomes underwent multiple rounds of manual refinement following automated binning, as described in the anvi'o pipeline.
• Raw sequencing reads are publicly accessible via the NCBI Sequence Read Archive under BioProject PRJNA1463332.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• The total number of genomes (rows) is unknown, which may limit suitability assessment.
• Data may reflect geographic bias inherent to the single aquifer system studied in Bahia, Brazil.

Provenance

Source

figshare, author Renato Romano.

Collection Method

Shotgun metagenomic sequencing of groundwater samples, followed by assembly with MEGAHIT, binning with CONCOCT and anvi'o, and quality assessment with CheckM.

Freshness

Last updated 2026-05-09 12:25:37; freshness should be verified.

Geography

Carbonate karst aquifer in the Irecê Basin, Bahia, northeastern Brazil.