A novel bacteriophage, P018-4, was isolated from Litopenaeus vannamei and characterized for its potential as a biocontrol agent. The phage's 126,958 bp circular double-stranded DNA genome contains 227 predicted ORFs, including ORF139 encoding a novel endolysin. Both the phage and its derived endolysins demonstrated bactericidal activity and biofilm disruption against the foodborne pathogen Vibrio parahaemolyticus.
Use Cases
- Evaluating phage stability based on temperature (4-70 °C) and pH (3-11) ranges
- Assessing bactericidal efficacy using multiplicity of infection (MOI) values of 0.001, 0.01, and 1
- Testing endolysin potency against Vibrio parahaemolyticus at concentrations like 0.135 mg/mL
- Analyzing biofilm disruption capabilities of phage-derived endolysins
Strengths
- Phage stability tested across a wide temperature range (4-70 °C) and pH levels (3-11)
- Genomic analysis provides a complete 126,958 bp circular double-stranded DNA sequence with 227 predicted ORFs
- Endolysin bactericidal activity quantified at a specific concentration (0.135 mg/mL)
Limitations
- Row count is unknown, which may limit suitability assessment
- Column-level documentation is absent; field semantics must be inferred after download
- The dataset is small (58.6 KB), indicating limited scope
Provenance
- Source
- Ruibing Yang via figshare
- Collection Method
- Experimental isolation and characterization of a bacteriophage and its derived endolysin.
- Freshness
- Last updated 2026-05-29 09:03:30; freshness should be verified