Lys-C digestion at 4 °C kept total asparagine modification levels below 4% under all tested conditions, providing a reliable strategy for LC–MS peptide mapping. Chengy Tan authored this dataset, which was last updated on May 8, 2026. The data likely contains experimental results comparing protease digestion methods and their effects on artificial deamidation.
Use Cases
- Compare protease digestion protocols based on temperature, pH, and time parameters mentioned in the description
- Evaluate artificial deamidation reduction strategies based on reported modification levels
- Optimize peptide mapping workflows for monoclonal antibodies based on the described design of experiments approach
Strengths
- File size is 20.1 KB, indicating a focused and manageable dataset
- License is CC0-1.0, allowing unrestricted use
- The description provides specific quantitative results, such as modification levels of 4% and 11.3%
Limitations
- Row count is unknown, which may limit suitability assessment
- Column-level documentation is absent; field semantics must be inferred after download
- The dataset is very small (20.1 KB), indicating limited scope
Provenance
- Source
- figshare
- Collection Method
- A design of experiments (DoE) approach was used to evaluate the effects of temperature, pH, and digestion time on modification levels.
- Freshness
- Last updated 2026-05-08 06:58:07; freshness should be verified