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A 229-sample calibration dataset from 28 temperate lakes provides the first direct comparison of lake-depth transfer functions based on testate amoebae species versus their physical traits. Taxonomy-based reconstructions captured latitudinal climate signals, while morphotrait-based reconstructions responded to local depth and trophic status independent of latitude. Both models performed well in littoral and metalimnetic zones but failed in profundal zones where environmental gradients weaken.
License is CC-BY-4.0. Primary data format is XLSX.