PKN2 Knockout Mouse Hearts: Protein-Level Differential Abundance Results

Quantitative proteomics results from embryonic mouse hearts comparing PKN2 cardiomyocyte-specific knockout and control genotypes. The dataset contains protein identifiers, fold-change estimates, and statistical significance values derived from TMT-based LC–MS/MS analysis. It was authored by Julijus Bogomolovas and last updated on 2026-05-05.

Use Cases

• Identify proteins significantly altered in PKN2 knockout hearts based on the `significant` and `cKO_vs_Control_significant` logical indicators.
• Calculate fold-change ratios of protein abundance using the `cKO_vs_Control_ratio` column.
• Perform downstream pathway analysis based on the list of differentially abundant proteins identified by gene symbol (`name`) and UniProt ID (`ID`).
• Validate statistical significance thresholds using the provided raw (`cKO_vs_Control_p.val`) and adjusted (`cKO_vs_Control_p.adj`) p-values.

Strengths

• Includes both raw and false discovery rate-adjusted p-values for statistical rigor.
• Provides mean-centered normalized abundance values for control and knockout samples for relative comparison.
• File size is 650.2 KB, indicating a manageable download and processing scope.

Limitations

• Row count is unknown, which may limit suitability assessment for large-scale analyses.
• Data is derived from pooled ventricular samples, which may obscure individual variability.
• Freshness should be verified; the last update date is 2026-05-05.

Provenance

Source

figshare

Collection Method

Quantitative mass spectrometry–based proteomics using TMT-based LC–MS/MS on pooled ventricular samples.

Freshness

2026-05-05