Blastocystis Genotyping Data from PCR-RFLP Analysis of 42 Human Samples

42 human fecal samples positive for Blastocystis spp. were analyzed using standardized PCR-RFLP methods. The dataset likely contains results from comparing DNA extraction methods, amplification efficiency, and subtype distribution. It was created by Maria Alejandra Vethencourt Ysea and last updated in May 2026.

Use Cases

• Compare DNA extraction method performance based on the Wizard®, Wizard Genomic®, and DNAzol® methods tested.
• Analyze subtype prevalence and mixed infections based on the reported ST1 (45%), ST3 (30%), ST4 (10%), and mixed infection (15%) frequencies.
• Evaluate amplification efficiency across sample types based on the reported 87.5% success in cultures and 68.2% in washed stool.
• Standardize molecular diagnostic protocols for Blastocystis based on the described PCR-RFLP method using Hinf I and Rsa I enzymes.

Strengths

• Includes results from 42 individual samples, providing a defined sample size.
• Compares three specific DNA extraction methods (Wizard®, Wizard Genomic®, DNAzol®) with a reported best performer.
• Reports specific subtype prevalence percentages: ST1 (45%), ST3 (30%), ST4 (10%), and mixed infections (15%).

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is 39.3 KB, indicating a very limited scope.

Provenance

Source

figshare

Collection Method

Descriptive study using microscopy, concentration techniques, culture, and molecular analysis (PCR-RFLP).

Freshness

Last updated 2026-05-05 16:53:46; freshness should be verified.