RR-10 Mission: Bulk and Spatial RNA Sequencing of Mouse Cerebellums in Spaceflight

NASA's Rodent Research-10 mission investigated bone regeneration in space using 20 female mice flown to the ISS for 28-29 days. This dataset includes bulk RNA sequencing and spatially resolved transcriptional profiling data from the cerebellums of 5 Wild Type flight animals and 5 Wild Type ground control animals. Samples were processed post-flight from carcasses preserved at -80°C or colder.

Use Cases

• Identify differential gene expression in the cerebellum due to spaceflight based on bulk RNA-seq data.
• Analyze spatial patterns of gene activity within cerebellar tissue sections using spatially resolved transcriptional profiling.
• Compare molecular responses between spaceflight and ground control groups (Basal, Vivarium, ISSES) described in the study.
• Investigate the impact of the p21-null genetic strain versus Wild Type mice on spaceflight adaptation, as referenced in the full experimental design.

Strengths

• Data originates from a controlled NASA spaceflight experiment (RR-10 mission) with defined ground control groups.
• Includes two complementary molecular profiling techniques: bulk RNA sequencing and spatially resolved transcriptional profiling.
• Experimental timeline and animal treatments (e.g., Alizarin Red, Calcein, BrdU injections) are precisely documented.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset subset includes only 10 animals (5 flight, 5 control WT); data from p21-null mice and other control groups may not be included.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Bulk RNA sequencing and spatial transcriptomics performed on cryosectioned cerebellums from mice flown on the SpaceX-21 mission to the ISS.

Time Range

Experimental period involved 28-29 days of microgravity exposure.

Freshness

Last updated 2026-03-13 19:52:04.537857; freshness should be verified.

Geography

Samples from mice flown on the International Space Station, with ground controls from simulated environments.