CD47-Purified Human Stem Cell-Derived Ventricular Cardiomyocytes for Drug Testing

A dataset from figshare describes a method for purifying ventricular-like cardiomyocytes from human pluripotent stem cells using CD47-based cell sorting. The dataset, created by Soon-Jung Park and last updated in April 2026, includes DOCX and MP4 files totaling 78.2 MB. It documents the enhanced maturity and drug responsiveness of the purified cells, validated through immunocytochemistry, patch clamp, and multi-electrode array recordings.

Use Cases

• Validate cardiac drug toxicity based on field potential alterations measured by multi-electrode array recordings mentioned in the description.
• Study ventricular action potential waveforms based on electrophysiological analysis using patch clamp techniques described.
• Assess cardiomyocyte structural maturation based on immunocytochemistry for myosin regulatory light chain 2 and α-actinin referenced.
• Compare purification methods based on the sensitivity and inter-replicate consistency analysis of CD47-enriched cells versus metabolically purified controls.

Strengths

• Includes multimodal documentation with DOCX and MP4 files totaling 78.2 MB.
• Method validated using three analytical techniques: immunocytochemistry, patch clamp, and multi-electrode array recordings.
• Drug responsiveness was tested with known cardiotoxic agents remdesivir and quinidine, showing dose-dependent effects.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset size of 78.2 MB is relatively small, potentially limiting the volume of raw experimental data.

Provenance

Source

figshare

Collection Method

Derived from human pluripotent stem cells differentiated into 3D spheroids and purified via CD47-based fluorescence-activated cell sorting.

Freshness

Last updated 2026-04-21 04:16:15; freshness should be verified.