White-Tailed Eagle Erythrocyte microRNA Sequencing Data from Norway, 2016

Two white-tailed eagle nestling erythrocyte samples from Steigen, Norway (67.93°N, 14.98°E) in 2016 were sequenced for small RNA analysis. The dataset includes processed outputs like miRTrace quality control reports and miRDeep2 quantification results for avian microRNAs. Raw sequencing data are available under ENA accession PRJEB73558.

Use Cases

• Quantifying known avian microRNA expression in erythrocytes based on reference-based annotation mentioned in the description.
• Assessing cross-species contamination in small RNA sequencing data using the provided miRTrace quality control reports.
• Investigating the influence of hemolysis on circulating microRNA measurements for biomarker studies in wild birds.
• Benchmarking quality-control procedures for microRNA sequencing, particularly for field-collected samples.

Strengths

• Includes detailed documentation of laboratory procedures and data analysis in the README file.
• Provides specific contamination metrics, with only 20.0% and 58.1% of classified microRNA reads assigned to birds in the two samples.
• Links to publicly available raw sequencing data under a known accession (PRJEB73558).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Dataset is based on only two biological samples from a single location and year.

Provenance

Source

European Nucleotide Archive (ENA) under accession PRJEB73558, with sample identifiers ERS18389117 and ERS18389118.

Collection Method

Small RNA sequencing of erythrocytes from two white-tailed eagle nestlings, with reference-based annotation.

Time Range

2016

Freshness

Last updated 2026-07-12 10:10:39; freshness should be verified.

Geography

Steigen, Norway (67.93°N, 14.98°E)