APEX04-EPEX: Arabidopsis RNA-Seq and MethylSeq Data from Spaceflight Experiment

RNA-Seq and MethylSeq data from an ISS experiment investigating epigenetic changes in Arabidopsis thaliana mutants under spaceflight stress. The National Aeronautics and Space Administration conducted the APEX04-EPEX experiment, comparing genome-wide methylation and gene expression profiles of two methylation-regulating gene mutants (met1-7 and elp2-5) and a wild type control, grown both on the ISS and on the ground. Data includes organ-specific analyses for leaf and root tissues to investigate differential gene expression patterns.

Use Cases

• Identify differentially expressed genes in response to spaceflight stress based on RNA-Seq data.
• Compare genome-wide methylation profiles between mutant and wild-type plants under spaceflight conditions.
• Investigate organ-specific epigenetic effects contributing to gene expression differences between leaf and root tissues.
• Analyze the role of 5mCyt and specific methylation regulators (met1-7, elp2-5) in plant adaptation to environmental stressors.

Strengths

• Data originates from a controlled experiment conducted on the International Space Station (ISS).
• Includes both spaceflight-grown samples and ground-grown controls for comparison.
• Analyses were performed in an organ-specific manner for leaf and root tissues.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

MethylSeq and RNAseq analyses performed on Arabidopsis thaliana samples from the APEX04-EPEX ISS experiment.

Time Range

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Freshness

Last updated 2026-03-13 19:48:21.646701; freshness should be verified.

Geography

International Space Station and ground controls.