Kronos Wheat: Chromosome-Level Genome Assembly and lncRNA Annotation

526 Gbp of high-fidelity reads were assembled and scaffolded with Hi-C data to produce a haplotype-collapsed genome assembly for the durum wheat variety 'Kronos'. The resulting 14 scaffolds, each greater than 600 Mbp, represent the 14 chromosomes of this tetraploid wheat (7 x AB). An updated long non-coding RNA (lncRNA) annotation (v1.1) is included, where 150 outlier genes were filtered out.

Use Cases

• Comparative genomics of polyploid wheat species based on the chromosome-level assembly.
• Identification and functional analysis of long non-coding RNAs (lncRNAs) in durum wheat.
• Genome-wide association studies (GWAS) using the high-quality reference genome for trait mapping.
• Evolutionary analysis of the AB subgenomes in tetraploid wheat.

Strengths

• Assembly is based on 526 Gbp of high-fidelity (HiFi) sequencing reads, indicating high base accuracy.
• Scaffolding with Hi-C data produced 14 chromosome-scale scaffolds (>600 Mbp each), representing a complete chromosome-level assembly.
• lncRNA annotation has been curated, with a specific version (v1.1) that removed 150 outlier genes based on statistical filtering.

Limitations

• Critical metadata such as row count, column names, file size, and specific update dates are missing from all platform entries.
• The data is shared under a restrictive prepublication Toronto Agreement, limiting redistribution and requiring coordination with authors for publication.
• The dataset's availability and structure are described textually, but the exact file formats and data organization are not specified.

Provenance

Source

University of California, Berkeley (contact: Ksenia Krasileva).

Collection Method

High-fidelity (HiFi) read sequencing, assembly with hifiasm, and scaffolding with Hi-C data using yahs.