Gryllus bimaculatus: Chromosome-Scale Genome Assembly and Annotation

Gryllus bimaculatus, the two-spotted cricket, is a key hemimetabolous model organism for developmental biology, neuroscience, and regeneration. This dataset provides a chromosome-scale nuclear genome assembly, mitochondrial genome sequence, and structural and functional annotation files for the white-eyed mutant strain. The assembly was generated using a hybrid strategy combining Oxford Nanopore, PacBio HiFi, Illumina, and Hi-C scaffolding, representing a major improvement over a previous 2021 draft.

Use Cases

• Comparative genomics analyses based on the chromosome-scale assembly and gene annotation.
• Neuroscience research leveraging the manually curated neuropeptide annotation patch.
• Functional annotation of genes using the provided gene models, CDS, and protein sequences.
• Studying genome evolution and structure using the Hi-C scaffolded assembly.

Strengths

• Assembly is chromosome-scale, generated via a hybrid long-read and Hi-C scaffolding strategy.
• Includes a manually curated neuropeptide annotation patch for five specific genes.
• Dataset is actively maintained, with updates for identifier consistency and new annotations in 2026.

Limitations

• Specific row counts and a complete list of data columns are not provided in the available metadata.
• The dataset's size (1.78 GB) suggests it is large, but the exact file composition and structure are not detailed.

Provenance

Source

Kosuke Kataoka

Collection Method

Hybrid genome assembly combining Oxford Nanopore, PacBio HiFi, Illumina short-read polishing, and Hi-C scaffolding.

Freshness

Last updated on 2026-05-26 with a neuropeptide annotation patch.