Co-release of GABA and ACh from Medial Olivocochlear Terminals in Mouse Cochlea

30.9 MB of experimental data from a study exploring neurotransmitter co-release at the medial olivocochlear (MOC) to inner hair cell (IHC) synapse in mice. The dataset, created by Tais Castagnola and last updated in April 2026, includes results from optogenetic stimulation, optical neurotransmitter detection, and calcium imaging. Findings indicate GABA and acetylcholine are co-released from at least a subset of MOC terminals, with GABA acting as a negative feedback mechanism during an immature developmental stage.

Use Cases

• Analyzing heterogeneity in GABAergic modulation based on the finding that only 15 out of 31 recorded synaptic sites were affected by a GABAB receptor antagonist.
• Studying synaptic currents in inner hair cells evoked by optogenetic stimulation of MOC terminals from GAD;ChR2-eYFP and ChAT;ChR2-eYFP mice.
• Investigating the role of GABAB receptors as a negative feedback mechanism regulating cholinergic inhibition at efferent→IHC synapses.
• Examining the co-release of GABA and acetylcholine from cholinergic fibers using pharmacological blockers like α-bungarotoxin and CGP 35348.

Strengths

• Data includes results from multiple experimental techniques: optogenetics, calcium imaging, and optical neurotransmitter detection.
• Specific quantitative findings are reported, such as 15 out of 31 synaptic sites showing GABA modulation.
• Dataset is 30.9 MB in size and available under a permissive CC-BY-4.0 license.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare, author Tais Castagnola.

Collection Method

Experimental data gathered via optogenetic stimulation, calcium imaging, and optical neurotransmitter detection in mouse cochlea.

Freshness

Last updated 2026-04-13 23:12:09; freshness should be verified.