Benzodiazepine Protein Target Data from Rat Brain Synaptosomes

Affinity-based protein profiling data identifies benzodiazepine drug targets in rat brain synaptosomes. The dataset includes results from three synthesized benzodiazepine affinity-based probes, with minimal overlap in identified protein targets between probes. It was generated using the chemoprotR R package for combined quantitative and qualitative statistical analysis of chemoproteomic data.

Use Cases

• Compare protein target identification results across the three synthesized benzodiazepine affinity-based probes to assess probe specificity.
• Apply combined statistical analyses from the chemoprotR package to interpret presence-absence patterns in label-free affinity-based protein profiling data.
• Identify GABA_A receptor subunits and proteins with ion channel functions as targets of benzodiazepine affinity-based probes.
• Analyze competitive labeling results with flurazepam to distinguish specific protein binders from non-specific interactions.

Strengths

• Data generated from rat brain synaptosomes, a complex biological sample relevant for neuropharmacology.
• Includes results from three distinct benzodiazepine affinity-based probes for comparative analysis.
• Analyzed with a dedicated R package (chemoprotR) for chemoproteomic data, ensuring methodological consistency.

Limitations

• Sample size and number of protein targets are unspecified, limiting assessment of statistical power.
• Data is derived from a single model organism (rat), which may not fully translate to human biology.
• The 2.4 MB file size suggests a relatively small dataset, potentially containing limited observations.

Provenance

Source

Lydia H. Griggs via figshare.

Collection Method

Affinity-based protein profiling (AfBPP) applied to rat brain synaptosomes using synthesized benzodiazepine probes, with competitive labeling experiments.

Time Range

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Freshness

Last updated March 2026.

Geography

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