Benzodiazepine Protein Target Data from Rat Brain Synaptosomes

Rat brain synaptosome data from affinity-based protein profiling experiments identifies benzodiazepine drug targets. The dataset supports the chemoprotR R package for combined quantitative and qualitative analysis of chemoproteomic data with high missingness. It contains results from three synthesized benzodiazepine affinity-based probes applied to complex protein samples.

Use Cases

• Identify protein targets from affinity-based probe data using the chemoprotR package's statistical methods for presence–absence data.
• Analyze overlap in protein targets identified by competitive labeling with flurazepam across three different benzodiazepine probes.
• Compare the number of significant protein targets yielded by the FR-DA (flurazepam-based) probe versus probes based on flunitrazepam.
• Investigate benzodiazepine interactions with proteins beyond GABA_A receptor subunits, such as those with ion channel functions.

Strengths

• Data generated from synthesized benzodiazepine affinity-based probes applied to rat brain synaptosomes.
• Includes results from three distinct probes for comparative analysis.
• Accompanies a published R package (chemoprotR) for specialized statistical analysis of such data.
• Released under a CC BY-NC 4.0 license for clear reuse terms.

Limitations

• Dataset is small at 2.5 MB, indicating limited scope or sample depth.
• Inherent high missingness in label-free affinity-based protein profiling data, especially in negative controls.
• Specific row count, column names, and sample data are unavailable, hindering preliminary assessment.

Provenance

Source

Lydia H. Griggs via figshare

Collection Method

Affinity-based protein profiling (AfBPP) using synthesized benzodiazepine probes on rat brain synaptosomes, with competitive labeling experiments.

Time Range

null

Freshness

Last updated March 14, -2026.

Geography

null