Human Monocyte-Derived Macrophage Lipidomic Profiles During 5-Day Differentiation

A lipidomic analysis of human primary blood monocytes differentiated for 5 days with M-CSF or GM-CSF. The dataset, authored by Alice Bacon and updated in May 2026, contains profiles of approximately 400 lipids across 16 classes, quantified using differential mobility spectrometry–shotgun lipidomics assistant (DMS-SLA). Spectral flow cytometry data on metabolic enzyme expression is also included.

Use Cases

• Modeling lipid metabolic dynamics based on the quantification of ~400 lipids across 16 classes.
• Comparing lipid profiles between M-CSF and GM-CSF derived macrophages based on described differences in FA 22:6 incorporation and enzyme expression.
• Analyzing the relationship between metabolic enzyme expression (e.g., G6PD, SDHA) and lipid species remodeling during differentiation.
• Investigating shifts in acyl chain length and unsaturation in triglyceride and phosphatidylcholine species during monocyte-to-macrophage transition.

Strengths

• Quantifies approximately 400 distinct lipid species across 16 lipid classes.
• Includes paired lipidomic and spectral flow cytometry data for metabolic enzyme expression.
• Compares two distinct differentiation conditions (M-CSF vs. GM-CSF) over a 5-day time course.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (74.8 KB), indicating a limited scope likely focused on a specific experimental cohort.

Provenance

Source

Alice Bacon via figshare.

Collection Method

Primary blood monocytes were differentiated in vitro for 5 days. Lipidomic profiling used the DMS-SLA platform, and spectral flow cytometry assessed enzyme expression.

Time Range

The experimental time course covers a 5-day differentiation period.

Freshness

Last updated 2026-05-28 23:46:57; freshness should be verified.