Alternative Splicing Profiles in a Rat Neuropathic Pain Model with THP Treatment

Jun Zhou analyzed 12 RNA-seq datasets from a rat model of neuropathic pain. The data profiles alternative splicing in Sham, chronic constriction injury (CCI), and CCI treated with L-tetrahydropalmatine (CCI_THP) groups. Findings were validated through behavioural testing, RT-qPCR, and immunohistochemistry.

Use Cases

• Identify differentially spliced genes in neuropathic pain based on RNA-seq data from three experimental groups.
• Analyze co-expression networks of RNA-binding proteins (RBPs) based on splicing-associated RBP networks mentioned in the description.
• Validate potential therapeutic targets for pain relief based on splicing alterations reversed by THP treatment.
• Investigate synaptic remodelling pathways based on enrichment of differentially spliced genes in axonogenesis and synapse assembly.

Strengths

• Data is derived from 12 RNA-seq datasets (GSE217932), providing a transcriptome-wide view.
• Key findings were validated in vivo using multiple methods, including behavioural testing and immunofluorescence.
• Dataset is licensed under CC-BY-4.0, allowing for open sharing and reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 1.3 MB size suggests the downloadable file is a PDF summary, not the primary sequence data.

Provenance

Source

figshare, author Jun Zhou.

Collection Method

RNA-seq analysis using the SUVA pipeline on samples from a rat chronic constriction injury model.

Freshness

Last updated 2026-06-03 08:56:07; freshness should be verified.