Cas9-sgRNA-DNA Ternary Complex Conformational States with Magnesium

Xing Zhu from the National Institutes of Health provides data on the conformational states of the Cas9-sgRNA-DNA ternary complex. The description details structural transitions, including a ~34-Å swing of the HNH nuclease domain between precatalytic and postcatalytic states. This data offers insights into the molecular mechanism of CRISPR-Cas9 genome editing.

Use Cases

• Modeling protein domain motions based on described conformational states like the 'checkpoint' and postcatalytic states.
• Simulating enzyme-nucleic acid interactions based on described interactions of REC3 and RuvC domains.
• Analyzing the structural mechanism of DNA cleavage based on the described HNH domain swing and active site details.

Strengths

• Data is associated with research from the National Institutes of Health, a major public health research institution.
• The description provides specific structural details, such as a ~34-Å swing of the HNH domain.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

National Institutes of Health