Continuous Imaging of Tumor Spheroids in 3D Collagen ECM

Continuous Imaging data captures tumor spheroids embedded in a 3D type I collagen extracellular matrix with photocrosslinked patches. Imaging was performed on a Leica TCS SPE confocal microscope using a 10x air objective at 0.40 NA for a 24-hour period, acquiring one frame every 15 minutes. The dataset is provided by author Bo Sun under a CC BY 4.0 license.

Use Cases

• Analyze cell migration and invasion patterns from spheroid images within the 3D collagen ECM over the 24-hour time-lapse.
• Quantify morphological changes in tumor spheroids interacting with the photocrosslinked ECM patches captured in each frame.
• Train a segmentation model to identify spheroid boundaries and ECM structures from the continuous confocal microscope images.

Strengths

• Provides 24 hours of continuous imaging data with a consistent 15-minute frame interval.
• Imaging performed with a specific Leica TCS SPE confocal microscope and a 10x air, 0.40 NA objective, ensuring methodological consistency.
• Dataset size of 998.9 MB indicates substantial visual data volume for analysis.

Limitations

• The specific number of images, rows, or columns is unknown, complicating project planning and computational resource estimation.
• Data is confined to a single, controlled experimental setup (3D type I collagen ECM with patches), limiting generalizability to other microenvironments.

Provenance

Source

figshare, author Bo Sun.

Collection Method

Imaging done on a Leica TCS SPE confocal microscope with a 10x air, 0.40 NA objective.

Time Range

24-hour imaging period.

Freshness

Last updated March 13, 2026.

Geography

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