Covalent: Protein Structures for Siderophore Transporter Identification

A collection of protein structure files (PDB format) detailing covalent tagging strategies for transmembrane transport proteins. The dataset, authored by Minhua Cao and last updated in May 2026, includes co-crystal structures of designed probes bound to transporters like FoxA, FpvB, FhuA, and FhuE in bacteria such as Pseudomonas aeruginosa and Escherichia coli.

Use Cases

• Analyzing protein-ligand interactions based on the co-crystal structure of Fe-D1 bound to FoxA.
• Studying transporter specificity and promiscuity based on the identification of proteins tagged by DFO-azir-06.
• Designing new covalent photo-cross-linker probes based on the described rational design approach.
• Investigating the role of cobalamine transporter BtuB in DFO derivative transport, as revealed by DFO-azir-06.

Strengths

• Includes specific, experimentally determined co-crystal structures (e.g., Fe-D1 bound to FoxA).
• Describes successful application of designed probes (DFO-azir-05, DFO-azir-06) to tag multiple known transporters (FoxA, FpvB, FhuA, FhuE).
• Dataset is small (873.3 KB), facilitating quick download and inspection.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect experimental bias inherent to the specific bacterial strains and probes used.

Provenance

Source

figshare

Collection Method

Rational design of covalent photo-cross-linking siderophore conjugates and structural analysis.

Freshness

Last updated 2026-05-13 18:36:05; freshness should be verified.