Covalent: Bacterial Transmembrane Transporters Tagged with Siderophore Probes

A dataset from 2026 by Minhua Cao, shared via figshare under a CC-BY-NC-4.0 license. It contains results from a covalent tagging strategy used to directly identify bacterial transmembrane proteins involved in siderophore-mediated iron uptake. The data likely includes protein identification results from experiments using designed photo-cross-linker probes on organisms like Pseudomonas aeruginosa and Escherichia coli.

Use Cases

• Identify bacterial transporters for ferrioxamine siderophores based on covalent tagging data.
• Study protein-ligand interactions in siderophore-metal complexes based on the described probe design.
• Investigate promiscuous or lower-affinity transport systems in bacteria based on the direct tagging methodology.
• Validate or discover roles for known transporters like BtuB in siderophore derivative transport.

Strengths

• Data is derived from a direct covalent tagging method, which can reveal lower-affinity transporters missed by indirect methods.
• Probes were rationally designed based on co-crystal structures, suggesting a targeted experimental approach.
• Includes results from multiple bacterial species (Pseudomonas aeruginosa, Escherichia coli K-12).

Limitations

• Dataset is small (98.4 KB), indicating limited scope or a focused subset of results.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

Minhua Cao via figshare.

Collection Method

Experimental results from covalent photo-cross-linking siderophore conjugates applied to bacterial cultures.

Freshness

Last updated 2026-05-13 18:36:07; freshness should be verified.