CRISPR-Cas9 Screens: Reformatted Genome-Wide Single-Guide RNA Files

Reformatted raw single-guide RNA files from genome-wide pooled CRISPR-Cas9 screens published by Behan F.M. et al. in Nature, 2019. The dataset, authored by Alessandro Vinceti, is a 1.0 GB collection of GZ files. It was last updated on May 4, 2026, and is shared under a CC0 1.0 license.

Use Cases

• Identify essential genes based on depletion of specific single-guide RNAs.
• Analyze gene knockout fitness effects across different cell lines or conditions.
• Validate or compare results from the original Nature 2019 study using the reformatted raw data.
• Develop or benchmark computational pipelines for processing CRISPR screen output files.

Strengths

• Data originates from a peer-reviewed study published in Nature, 2019.
• The 1.0 GB size suggests a substantial volume of raw screen data.
• Released under a CC0 1.0 license, allowing for maximum reuse and redistribution.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for specific analyses.
• The description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

Behan F.M. et al., Nature, 2019.

Collection Method

Reformatted raw data from genome-wide pooled CRISPR-Cas9 screens.

Time Range

Associated with the 2019 publication.

Freshness

Last updated 2026 05 04 16:54:58; freshness should be verified.

Geography

null