CRISPRi Repression Efficiency and Toxicity Across Four Escherichia coli Strains

Experimental data from a 2026 study by Hyerim Ban investigating the transferability of CRISPR interference systems. The dataset compares repression efficiency and cytotoxicity of three CRISPRi systems across four E. coli strains, including a laboratory K-12 strain and three clinical isolates. It includes results for guide RNA targeting and the development of a dual gRNA system achieving up to 824-fold repression.

Use Cases

• Compare CRISPRi repression efficiency based on results from guide RNA targeting along gene sequences.
• Assess host strain toxicity based on growth toxicity measurements from dCas protein expression.
• Design enhanced gene silencing systems based on the dual gRNA CRISPRi system results.
• Evaluate strain-specific design considerations for transferring genetic tools between laboratory and clinical isolates.

Strengths

• Includes data from three distinct CRISPRi systems (one dCas9 and two dCas12a variants).
• Compares results across four different E. coli strains, including three clinical isolates.
• Reports a specific result of up to 824-fold repression achieved in the CFT073 strain.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small at 168.3 KB, indicating limited scope.

Provenance

Source

figshare

Collection Method

Laboratory experiments evaluating repression efficiency and cytotoxicity.

Freshness

Last updated 2026-05-02 17:08:20; freshness should be verified.