CRISPRi Repression Efficiency and Toxicity Across Four Escherichia coli Strains

A 13.9 KB dataset by Hyerim Ban, last updated May 2, 2026, on figshare. It investigates the repression efficiency and cytotoxicity of three CRISPRi systems (dCas9 and two dCas12a variants) across four E. coli strains, including laboratory and clinical isolates. The study developed a dual gRNA system achieving up to 824-fold repression in one strain.

Use Cases

• Compare CRISPRi system performance based on repression efficiency data across different bacterial strains.
• Assess host toxicity based on growth toxicity measurements from dCas protein expression.
• Design enhanced gene silencing strategies based on the dual gRNA system results.
• Evaluate strain-specific design rules for transferring genetic tools from laboratory to clinical isolates.

Strengths

• Compares three distinct CRISPRi systems (dCas9 and two dCas12a variants) across four different E. coli strains.
• Includes quantitative results such as up to 824-fold repression achieved in the CFT073 strain.
• Provides specific comparisons between a laboratory K-12 strain and three clinical isolates (Nissle 1917, CFT073, UMN026).

Limitations

• Dataset size is only 13.9 KB, indicating a very limited scope.
• Row count and column-level documentation are unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Experimental evaluation of CRISPRi systems in laboratory conditions.

Freshness

Last updated 2026-05-02 17:08:19; freshness should be verified.