CRISPRi Repression Efficiency and Toxicity Across Four Escherichia coli Strains

Cross-Strain Transferability of CRISPRi Systems and Design Rules from Laboratory to Clinical Escherichia coli Strains is a dataset from figshare. It contains results from an investigation of three CRISPRi systems (one dCas9 and two dCas12a variants) across four E. coli strains, including a laboratory K-12 strain and three clinical isolates. The dataset, last updated in May 2026, includes measurements of repression efficiency and cytotoxicity, with the dual gRNA system achieving up to 824-fold repression in one strain.

Use Cases

• Compare CRISPRi repression efficiency based on data from guide RNAs targeting along gene sequences across different strains.
• Assess host strain toxicity based on growth toxicity measurements from expressing different dCas proteins.
• Evaluate the performance of a dual gRNA CRISPRi system for enhanced gene silencing based on the reported 824-fold repression in CFT073.
• Investigate strain-specific design considerations for CRISPRi systems based on the variable repression observed between laboratory and clinical isolates.

Strengths

• Includes data from four distinct E. coli strains, specifically naming MG1655, Nissle 1917, CFT073, and UMN026.
• Compares three different CRISPRi systems (one dCas9 and two dCas12a variants) within the same study.
• Reports a specific quantitative result, with the dual gRNA system achieving up to 824-fold repression in the CFT073 strain.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small at 56.0 KB, indicating a limited scope of experimental results.

Provenance

Source

Hyerim Ban via figshare

Collection Method

Experimental evaluation of CRISPRi repression efficiency and cytotoxicity in laboratory and clinical E. coli strains.

Freshness

Last updated 2026-05-02 17:08:18; freshness should be verified.