CVA10: Cryo-EM Structures of Coxsackievirus A10 Mature Virus

Coxsackievirus A10 (CVA10) is a human enterovirus that can cause diseases ranging from hand-foot-and-mouth disease to polio-like illness. The dataset from Ling Zhu at the Chinese Academy of Sciences contains atomic-resolution cryo-electron microscopy structures of the CVA10 virion, an empty particle, and an uncoating intermediate, revealing conformational differences in receptor binding sites and epitopes compared to other virus subgroups. A medium-resolution genome structure is also included, reconstructed using a symmetry-mismatch method.

Use Cases

• Comparative structural analysis of KREMEN1-dependent versus SCARB2-dependent enterovirus subgroups based on receptor binding site conformations.
• Studying the virus uncoating process and RNA release mechanism based on the atomic-resolution structures of the mature virus, empty particle, and uncoating intermediate.
• Epitope mapping for vaccine design based on the serotype-specific epitope differences highlighted in the structural comparisons.
• Method development for cryo-EM reconstruction using the symmetry-mismatch method demonstrated for the genome structure.

Strengths

• Includes atomic-resolution structures of three distinct viral states: mature virion, empty particle, and uncoating intermediate.
• Structural comparisons reveal specific conformational differences in putative receptor binding sites and serotype-specific epitopes.
• Data originates from a research institution, the Chinese Academy of Sciences, and is associated with a published scientific finding.

Limitations

• Row count and dataset size are unknown, which may limit suitability assessment for computational tasks.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

Ling Zhu, Chinese Academy of Sciences

Collection Method

Cryo-electron microscopy and symmetry-mismatch reconstruction methods.