NifA Activation: CryoEM and X-ray Structural Data

Wenyao Zhang's dataset contains CryoEM density maps and X-ray crystallography structures for the transcriptional activator NifA, which is activated by 2-oxoglutarate for biological nitrogen fixation. The data supports structural studies of protein activation mechanisms. It is available under a CC-BY-4.0 license and is hosted on multiple platforms.

Use Cases

• Model building and refinement based on provided CryoEM density maps and atomic coordinates.
• Comparative structural analysis of NifA activation states using the X-ray crystallography structures.
• Studying ligand-protein interactions, specifically between NifA and the effector molecule 2-oxoglutarate.
• Methodological validation for integrative structural biology combining CryoEM and X-ray data.

Strengths

• Dataset size is substantial at approximately 1.75 GB, suggesting detailed structural data.
• Data is openly licensed under CC-BY-4.0, facilitating reuse and sharing.
• Presence on multiple platforms indicates community validation and importance.

Limitations

• Column names and a precise row count are not provided, limiting understanding of internal data structure.
• The dataset consists of compressed ZIP files, requiring extraction and potentially specialized software to interpret the structural formats.
• The listed update date of 2026-03 22 is in the future, creating uncertainty about the actual data freshness.

Provenance

Source

Wenyao Zhang

Collection Method

Experimental data from CryoEM and X-ray crystallography structural studies.

Time Range

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Freshness

Last updated date is listed as 2026-03-22 (future date, conflict noted).

Geography

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