CXCR5+ Monocyte Emigration: Gene Expression Profiles from Irradiated MC38 Tumors

6 tumor tissue samples from MC38 mouse models were used to generate gene expression profiles, comparing untreated tumors to those irradiated three or seven days prior. Sequencing libraries were prepared using the Hieff NGS Ultima Dual-mode mRNA Library Prep Kit, and transcripts were assembled with StringTie. The dataset, authored by Yuzhu Hou and last updated in May 2026, is shared under a CC-BY-4.0 license.

Use Cases

• Identify differentially expressed genes between untreated and irradiated tumors based on the described time-course comparison.
• Analyze transcriptomic differences between CXCR5+ and CXCR5- monocyte/macrophage subpopulations isolated via cell sorting.
• Investigate the role of monocyte-derived suppressor cells (M-MDSCs) in the tumor immune response based on their isolation from untreated tumors.

Strengths

• Includes gene expression data from 6 distinct tumor tissue samples.
• Provides comparisons across three experimental conditions (untreated, 3-day post-IR, 7-day post-IR).
• Describes a specific cell sorting protocol for isolating CXCR5+ and CXCR5- monocyte populations.

Limitations

• Row count and column-level documentation are unknown, limiting suitability assessment.
• The sample size is small, with only 2 replicates per experimental group.
• Data may reflect bias inherent to the specific mouse model and experimental protocol.

Provenance

Source

figshare

Collection Method

Gene expression profiling via RNA sequencing of sorted cell populations from mouse tumor models.

Freshness

Last updated 2026-05-13 07:03:15.