CyanOperon: Synthetic Operon Assembly Toolkit for Cyanobacteria

CyanOperon is an expansion of the CyanoGate MoClo toolkit for assembling synthetic operons, created by Michael J. Astbury and last updated on 2026-04-24. The system includes two Level 0 and 15 Level 1 acceptor vectors for hierarchical assembly and expression of up to six genes. It was showcased by assembling the violacein biosynthesis pathway and testing a 20-part RBS library in Escherichia coli and Synechocystis sp. PCC 6803.

Use Cases

• Designing and testing synthetic operons for coordinated gene expression based on the described hierarchical assembly system.
• Optimizing translation efficiency by screening ribosome binding site (RBS) libraries based on the 20-part RBS library mentioned.
• Comparing gene expression from chromosomal integration versus plasmid vectors based on the fluorescent marker experiments described.
• Integrating operon assemblies with other modular cloning (MoClo) systems for standardized genetic part workflows.

Strengths

• Includes 15 Level 1 acceptor vectors enabling assembly of operons with up to six genes.
• Demonstrated with a specific 20-part RBS library for translation optimization studies.
• Publicly available and designed for integration with other MoClo systems.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 68.5 KB, indicating limited scope.

Provenance

Source

Michael J. Astbury via figshare

Collection Method

Likely contains plasmid maps, sequence data, and experimental results from toolkit development and validation.

Time Range

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Freshness

Last updated 2026-04-24 14:07:13; freshness should be verified.

Geography

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