CyanOperon: Synthetic Operon Assembly Toolkit for Cyanobacteria and E. coli

A 47.5 KB toolkit published on figshare in April 2026 by Michael J. Astbury. The dataset describes the CyanOperon system, an expansion of the CyanoGate MoClo toolkit for building synthetic operons. It includes two Level 0 and 15 Level 1 acceptor vectors for assembling and expressing up to six genes within a single operon.

Use Cases

• Assembling multi-gene metabolic pathways, such as the violacein biosynthesis pathway demonstrated in the description
• Optimizing translation efficiency by constructing RBS libraries to examine Shine–Dalgarno sequence spacer effects
• Comparing gene expression from chromosomally integrated operons versus self-replicating vectors
• Integrating standardized operon assemblies with other modular cloning (MoClo) systems

Strengths

• Includes 15 Level 1 acceptor vectors for hierarchical assembly of up to six genes
• Demonstrated utility in two model organisms: E. coli and Synechocystis sp. PCC 6803
• Publicly available and designed for integration with other MoClo systems

Limitations

• Row count and column-level documentation are unknown; field semantics must be inferred after download
• The 47.5 KB size indicates a limited scope, likely containing protocol and vector maps rather than large experimental data

Provenance

Source

figshare

Collection Method

Toolkit development and experimental validation as described in the publication

Time Range

Publication date 2026-04-24

Freshness

Last updated 2026-04-24 14:07:14

Geography

Laboratory research, not geographically specific