Cytokine Production Profiles of ILC3 Subsets in NEC Mouse Model

Flow cytometry data from a 2026 study comparing cytokine production in intestinal immune cells between healthy mice and a mouse model of necrotizing enterocolitis (NEC). The dataset, authored by Junyu He, contains measurements of IL-17A and IL-22 producing cells within three distinct group 3 innate lymphoid cell (ILC3) subsets, with 6 biological replicates per experimental group.

Use Cases

• Compare cytokine production (IL-17A, IL-22) between healthy and disease-state mice based on the described experimental groups.
• Analyze functional differences between distinct ILC3 subsets (DN, CCR6+, NKp46+) based on the detection targets.
• Validate statistical comparisons (unpaired two-tailed t-test) between independent groups based on the described analysis.
• Benchmark flow cytometry protocols for intranuclear cytokine staining in intestinal lamina propria cells based on the listed antibodies and cytometer.

Strengths

• Experimental design includes 6 biological replicates per group, which may support statistical robustness.
• Specific detection targets are clearly defined, listing percentages for two cytokines across three ILC3 subsets.
• Statistical analysis method (unpaired two-tailed Student's t-test) and significance threshold (P < 0.05) are explicitly stated.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for specific analytical needs.
• Data may reflect bias inherent to a specific mouse strain (C57BL/6) and a standardized experimental induction procedure.

Provenance

Source

figshare, author Junyu He.

Collection Method

Data acquired via flow cytometry (CytoFLEX S) from intestinal lamina propria of wild-type C57BL/6 mice, with a subset subjected to experimental NEC induction.

Time Range

Study data likely corresponds to the publication/upload timeframe around 2026.

Freshness

Last updated 2026-04-16 03:07:06.

Geography

Laboratory study; geographic origin of the mice is not specified.