Cytometry, titration and PCR results following infection of Antigen-presenting Cells with

Results from a 2026 study comparing the infection of monocyte-derived macrophages and dendritic cells from goats, sheep, and cattle with two strains of peste des petits ruminants virus. The data includes flow cytometry percentages, virus titers (TCID50/mL), and RT-qPCR Cq values from a minimum of three animals per species and condition. The dataset was analyzed using generalized linear mixed models.

Use Cases

• Compare viral replication kinetics based on infection time points (24h, 48h, 72h, 96h) mentioned in the description.
• Analyze differences in infection percentages between cell types (MoMs vs. MoDCs) and animal species (goats, sheep, cattle).
• Model the relationship between viral strain virulence (MA08 vs. IC89) and measured outcomes (TCID50/mL, Cq values).
• Assess the immune response in different hosts using the flow cytometry data for infected cell percentages.

Strengths

• Data includes results from a minimum of three biological replicates per species and condition.
• Three distinct measurement types are integrated: flow cytometry percentages, virus titers, and RT-qPCR Cq values.
• Compares two PPRV strains with documented virulence differences (MA08 high virulence, IC89 low virulence).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last updated 2026-05-23 09:10:08; freshness should be verified.

Provenance

Source

CIRAD Harvested Collection, authored by Arnaud Bataille.

Collection Method

Experimental data from in vitro infections of differentiated immune cells, analyzed with generalized linear mixed models.

Freshness

Last updated 2026-05-23 09:10:08

Geography

Virus strains originated from Morocco (2008) and Ivory Coast (1989).