Porcine Polytrauma Model miRNA Expression in Lung Tissue

Featuring miRNA expression data from lung tissue samples of polytraumatized pigs, comparing two surgical treatment methods (Early Total Care and Damage Control Orthopedics) and a drug-based treatment group. The study involved 20 pigs across four experimental groups, analyzing miRNAs associated with lung function, inflammation, and fibrosis. Data was collected over a 72-hour monitoring period in an ICU setting.

Use Cases

• Analyze expression levels of anti-inflammatory miRNAs like miR-21, miR-23a, miR-27a, and miR-30b across DCO and ETC treatment groups.
• Correlate miRNA expression patterns with histological findings such as alveoli preservation, septal thickening, and inflammatory cell infiltration.
• Compare the effect of C5/CD14 inhibition therapy on inflammatory and fibrotic miRNA expression against standard surgical care groups.
• Investigate the regulatory role of specific miRNAs in post-traumatic innate immune responses within a porcine polytrauma model.

Strengths

• Data from a controlled porcine polytrauma model with four defined treatment groups (sham, ETC, DCO, ETC with C5/CD14 inhibition).
• Analysis includes both molecular (miRNA qPCR) and histological (PAS staining, in situ hybridization) data points.
• Study design includes 20 animals across groups, monitored for 72 hours in an ICU setting.

Limitations

• Small sample size per group (n=8 for ETC/DCO, n=4 for drug group), limiting statistical power for subgroup analyses.
• Data is derived from an animal model (porcine), which may not fully translate to human polytrauma pathophysiology.
• Dataset scope is limited to acute phase observations (72 hours), lacking long-term effects on tissue regeneration.

Provenance

Source

DataverseNL Harvested Dataverse, author von Münchhausen, Belinda.

Collection Method

Experimental data from a porcine polytrauma model involving blunt chest trauma, bilateral femur fractures, liver laceration, and hemorrhagic shock. miRNA isolation, transcription, qPCR analysis, and histological staining were performed on lung samples.

Time Range

Experimental monitoring period of 72 hours.

Freshness

Last updated 2026-03-02.