Toad Chemical Defences Across Larval and Post-Metamorphic Life Stages

An analytical chemistry dataset on bufadienolide compounds in common toads (Bufo bufo) created by Bálint Üveges. Data was generated using high-performance liquid chromatography with diode-array detection and mass spectrometry (HPLC-DAD-MS) with detailed methodological specifications. The dataset likely quantifies chemical defence changes dependent on age and environment.

Use Cases

• Modeling ontogenetic changes in chemical defence profiles based on life stage data.
• Analyzing correlations between environmental factors and bufadienolide compound concentrations.
• Developing reference libraries for mass spectrometry identification of amphibian toxins.

Strengths

• Detailed, reproducible analytical methodology using HPLC-DAD-MS is explicitly described.
• Identification of compounds was aided by multiple commercially acquired bufadienolide standards.
• Sample preparation steps, including homogenization, drying, and filtration, are fully specified.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count and dataset scale are unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

Bálint Üveges via paperswithcode

Collection Method

High-performance liquid chromatography with diode-array detection and mass spectrometry (HPLC-DAD-MS) analysis of homogenized toad specimens.