Coordination of Bacterial Cell Wall and Outer Membrane Biosynthesis Data

Raw data from a study on lipopolysaccharide biosynthesis regulation in gram-negative bacteria, including Pseudomonas aeruginosa. The dataset contains tarred directories for experimental microscopy and LC-MS/MS data, as well as covariation analysis files like multiple sequence alignments and AlphaFold structures. Associated analysis code is available on GitHub.

Use Cases

• Analyze protein covariation and interaction networks based on EVcomplex models and couplings.
• Study the regulation of the LpxC enzyme across diverse gram-negative bacteria using phylogenetic trees and sequence alignments.
• Validate structural models of biosynthetic proteins using provided AlphaFold structures.
• Correlate cellular localization patterns from microscopy data with biochemical data from LC-MS/MS.

Strengths

• Data is directly associated with a published scientific paper from Harvard University.
• Includes multiple complementary data modalities such as microscopy, mass spectrometry, and computational models.
• Analysis code is provided in a public GitHub repository for reproducibility.

Limitations

• Row count, file size, and specific column definitions are unknown, limiting suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

Harvard University

Collection Method

Experimental data collection (microscopy, LC-MS/MS) and computational analysis (covariation, AlphaFold).