Hippocampal Synapse Electron Tomography for Control and LTP Conditions

Transmission electron tomographic datasets of hippocampal synapses from male Long-Evans rats. The data includes raw tilt images, aligned images, reconstructed volumes, and summary images, acquired from hippocampal slices under control and long-term potentiation (LTP) conditions. The dataset was published by Jae Hoon Jung and is hosted on the 3DEM public data repository.

Use Cases

• Analyze synaptic vesicle distribution and tethering based on reconstructed 3D volumes.
• Compare ultrastructural changes in synapses between control and LTP conditions.
• Validate or train segmentation algorithms for subcellular components in electron tomography data.
• Study the relationship between presynaptic architecture and neurotransmitter release probability.

Strengths

• Data includes five distinct file types, providing raw, processed, and visual representations.
• Tilt images were acquired at a 1-degree interval along a single axis, enabling detailed 3D reconstruction.
• Dataset is publicly available from a specialized repository (3DEM.org) for electron microscopy data.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is from a specific model (male Long-Evans rats) and brain region, which may limit generalizability.

Provenance

Source

Texas Data Repository Harvested Dataverse / 3DEM.org

Collection Method

Transmission electron tomography of hippocampal slices, with tilt-series acquisition and reconstruction using EM3D software.

Time Range

Dataset associated with publication 'Jung et al 2021'.

Freshness

Last updated 2024-03-18 07:29:53; freshness should be verified.

Geography

Data derived from rat hippocampus; specific lab location is not stated.