Peptidergic Neuron Identifications in Larval Annelid Connectome

Serial multiplex immunogold labeling (siGOLD) data used to identify peptidergic neurons within a full-body serial-section transmission electron microscopy (ssTEM) connectome of a larval Platynereis annelid. The method employed 11 neuropeptide antibodies to overlay chemical neuromodulatory maps onto synaptic connectivity data. The dataset was created by Réza Shahidi and published in 2020.

Use Cases

• Map neuropeptide expression, such as pigment-dispersing factor, onto reconstructed synaptic circuits from ssTEM data.
• Validate the scalability of the siGOLD method by analyzing results from 11 distinct neuropeptide antibody labels.
• Identify and reconstruct peptidergic circuitry involving specific sensory organs like the nuchal organs.
• Correlate immunogold labeling patterns from small subsets of sections with neuronal morphology across larger series.

Strengths

• Applies a novel serial multiplex immunogold labeling (siGOLD) method to a full-body larval dataset.
• Uses 11 distinct neuropeptide antibodies for molecular identification within a connectomic context.
• Enables direct correlation of chemical neuromodulator maps with synaptic connectivity maps.

Limitations

• The specific data format, size, and structure (e.g., rows, columns) are not described.
• The dataset is focused on a single model organism (the annelid Platynereis), limiting generalizability.
• Data is from a 2020 publication and may not reflect the most recent methodological advances.

Provenance

Source

Dryad

Collection Method

Serial multiplex immunogold labeling (siGOLD) and serial-section transmission electron microscopy (ssTEM) on larval Platynereis.

Freshness

Last updated 2020-06-24.