Mouse Cardiac Function Data from Remote Ischemic Conditioning Study

69 C57BL/6 mice were allocated to study the effects of remote ischemic conditioning (RIC) on cardiac autophagy signaling and infarct size. The experiment examined acute (15 minutes), delayed (24 hours), and chronic (9 days) phases of cardioprotection. Data includes measurements of infarct size, left ventricular end diastolic pressure (LVEDP), and developed pressure (LVDP), along with protein levels like AMPK, mTOR, LC3II/I, and Atg5.

Use Cases

• Analyze the correlation between infarct size reduction and changes in LVEDP and LVDP across the three treatment phases.
• Model the relationship between mTOR suppression levels and the expression of autophagosome proteins LC3II/I and Atg5 in chronic RIC groups.
• Compare AMPK level changes between the acute/first window (1W RIC) and delayed/second window (2W RIC) treatment groups.
• Investigate the pattern of autophagy signaling by examining protein level variations across the six experimental groups (1W control, 1W RIC, 2W control, etc.).

Strengths

• Data is derived from a controlled experimental study with 69 C57BL/6 mice, providing a defined sample size.
• Includes measurements across three distinct temporal phases of cardioprotection: acute, delayed, and chronic.
• Assesses multiple physiological and molecular endpoints, including infarct size, cardiac function (LVEDP, LVDP), and specific protein levels (AMPK, mTOR, LC3II/I, Atg5).

Limitations

• Sample size is limited to 69 mice, which may restrict the statistical power for detecting subtle effects or interactions.
• Data is specific to a single mouse strain (C57BL/6) and may not generalize to other species or genetic backgrounds.
• The temporal scope is confined to the experimental protocol's defined time points, lacking longer-term follow-up data.

Provenance

Source

Dryad digital repository, authored by Sagar Rohailla.

Collection Method

Experimental data from a controlled laboratory study using a Langendorff preparation on isolated mouse hearts.

Time Range

Experimental time points include 15 minutes, 24 hours, and 9 days post-conditioning.

Freshness

Data was last updated in June 2020.