All-Optical Recording and Stimulation of Retinal Neurons in Degeneration Mice

A collection of in vivo measurements of retinal neuron function in mice with photoreceptor degeneration (rd10). It documents restored visual responses in ganglion cell layer neurons expressing the red-shifted channelrhodopsin ChrimsonR over a six-week period following significant vision loss. The data was collected using high-resolution adaptive optics scanning light ophthalmoscopy to image fluorescence from the calcium sensor GCaMP6s.

Use Cases

• Analyze the time course of restored visual responses in ganglion cell layer neurons following therapeutic intervention.
• Study the relationship between fluorescence signals from the calcium sensor GCaMP6s and neuronal activity in retinal degeneration models.
• Evaluate the efficacy of the channelrhodopsin ChrimsonR for vision restoration in rd10 mice over a six-week period.
• Correlate adaptive optics imaging data with functional measurements of neuronal processing in the living retina.

Strengths

• Data captures a six-week longitudinal study of retinal function following intervention.
• Combines multiple advanced techniques: fluorescent calcium imaging, channelrhodopsin stimulation, and adaptive optics.
• Focuses on a specific and relevant disease model: mice with photoreceptor degeneration (rd10).

Limitations

• Sample size and number of data points (rows) are unknown, limiting statistical power assessment.
• Data is specific to a single animal model (rd10 mice), which may limit generalizability to other retinal diseases.
• The dataset's structure, available features (columns), and file formats are unspecified.

Provenance

Source

Dryad digital repository.

Collection Method

High-resolution adaptive optics scanning light ophthalmoscopy (AOSLO) used to image fluorescence from the calcium sensor GCaMP6s in living mice.

Time Range

Measurements taken over a six-week period.

Freshness

Last updated in June 2020.

Geography

Laboratory study; specific geographic location not provided.