Calcium Imaging Data for α7/α9 Nicotinic Acetylcholine Receptor Mutants
by Irina Shelukhina·Updated 6y ago
Available on 1 platform
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Description
Calcium imaging results from probing ligand-binding properties of human and rat α7 nicotinic acetylcholine receptor (nAChR) mutants. The data was generated by transient co-expression of α7/α9 nAChR mutants with chaperones and the Case12 calcium sensor, followed by pharmacological analysis using fluorescence microscopy or a FLIPR reader. It includes determined affinities for acetylcholine and epibatidine for wild-type receptors and specific mutants at positions 117–119, 184, 185, 187, and 189.
Use Cases
Compare acetylcholine and epibatidine affinity values for α7 nAChR mutants to identify key ligand-binding residues.
Analyze the effect of mutations at positions 187 and 119 on ligand affinity based on the reported strongest decrease.
Validate calcium imaging results from the Case12 sensor by cross-referencing with data from conventional indicator Fluo-4 and electrophysiology.
Investigate the pharmacological impact of the L119D mutation on α7 nAChR, given its differential effect on epibatidine versus acetylcholine affinity.
Strengths
Data is validated by two independent methods: electrophysiology and calcium imaging with the Fluo-4 indicator.
Affinities are determined for two specific ligands, acetylcholine and epibatidine, across multiple mutant constructs.
The study focuses on a defined set of amino acid positions (117–119, 184, 185, 187, 189) anticipated to be involved in ligand binding.
Limitations
The dataset size, including the number of experimental replicates or data points, is not specified.
Data is limited to specific α7 nAChR mutants and two ligands, not covering the full pharmacological profile of α9 nAChRs.
The experimental method relies on transient co-expression in neuroblastoma cells, which may not fully represent native receptor function.
Provenance
Source
Dryad digital repository.
Collection Method
Calcium imaging with genetically encoded sensor Case12, co-expressed with α7/α9 nAChR mutants and chaperones in neuroblastoma cells, analyzed via fluorescence microscope or FLIPR.
Freshness
Last updated in June 2020.
License is CC0 1.0 Universal (Public Domain Dedication). Associated research involves species Naja kaouthia and Xenopus laevis. Specific file formats and data structure are not described.