Multi-Drug Resistant Vibrio Cholerae Outbreak Data from Nepal

Dryad hosts data from a 2012 study of cholera outbreaks in Nepal. The dataset includes 28 Vibrio cholerae isolates from patients and surface water in three districts, analyzed for antimicrobial resistance and genetic markers. Research confirmed the strains were toxigenic, multi-drug resistant, and clonally related to outbreaks in Bangladesh and Haiti.

Use Cases

• Analyze antimicrobial resistance patterns to SXT, NA, and S across the 28 V. cholerae isolates.
• Study genetic markers like the ctx B-7 allele and tcpA point mutations to understand virulence and transmission.
• Compare PFGE patterns to investigate clonal relationships between Nepalese strains and those from Bangladesh and Haiti.
• Examine the presence of VSP-I and absence of specific VSP-II ORFs for genomic characterization of the outbreak strain.

Strengths

• Includes 28 genetically characterized V. cholerae isolates from a defined 2012 outbreak.
• Provides detailed molecular data on virulence factors, antimicrobial resistance, and genetic fingerprinting.
• Strains are linked to specific geographic origins: Kathmandu (n=12), Doti (n=9), Bajhang (n=1), and surface water (n=6).

Limitations

• Small sample size of 28 isolates limits statistical power for some analyses.
• Data is from a single year (2012) and may not represent current cholera strains or resistance patterns.
• Geographic coverage is limited to three districts in Nepal.

Provenance

Source

Sameer M. Dixit via Dryad.

Collection Method

V. cholerae isolates from patient stool and surface water were tested via antimicrobial assays, PCR, DNA sequencing, and PFGE.

Time Range

2012.

Freshness

Data reflects 2012 outbreaks; metadata was last updated in 2020.

Geography

Three districts in Nepal: Kathmandu, Doti, and Bajhang.