Data from: Combination of serial plating and long-read sequencing to detect and quantify l

Experimental data from a study combining serial plating with long-read sequencing to detect and quantify live Shiga toxin-producing Escherichia coli (STEC) in ground beef. The method, developed by the Department of Agriculture, was able to quantify STEC down to 1 colony-forming unit per gram. The dataset was last updated on March 13, 2026.

Use Cases

• Benchmarking pathogen quantification methods based on the described serial plating and sequencing protocol.
• Training models for virulence gene identification based on sequencing data from single colonies.
• Assessing the impact of sample temperature handling on bacterial recovery rates as described in the experiments.
• Improving food safety risk assessment models based on the method's quantification sensitivity.

Strengths

• Method demonstrated quantification sensitivity down to 1 colony-forming unit per gram.
• Data is associated with a specific, peer-reviewed experimental protocol described in the summary.
• Dataset is published under a permissive CC0-1.0 license.
• Last update recorded as March 13, 2026, indicating recent activity.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count and dataset scale are unknown, which may limit suitability assessment.
• The data appears to be from controlled laboratory experiments, which may limit direct applicability to field samples.

Provenance

Source

Department of Agriculture

Collection Method

Data generated from a laboratory study inoculating ground beef with STEC, followed by serial plating, colony selection, and long-read sequencing.

Time Range

The study timeframe is not specified.

Freshness

Last updated 2026-03-13 22:44:34.110745; freshness should be verified.

Geography

The geographic scope of the study is not specified.