Transient Adenosine Measurements During Rat Cerebral Ischemia-Reperfusion

Aggregating electrochemical measurements of transient adenosine release in the caudate-putamen of anesthetized rats. Data were collected over four hours, covering two hours of normoxia, 30 minutes of cerebral ischemia, and 90 minutes of reperfusion. The study quantified changes in transient frequency and concentration, including the effects of an A2A antagonist.

Use Cases

• Analyze the correlation between transient adenosine frequency and the cerebral ischemia-reperfusion phases to model temporal dynamics.
• Compare cumulative adenosine concentration during normoxia versus ischemia-reperfusion periods to quantify the 53% increase reported.
• Evaluate the impact of the A2A antagonist SCH442416 on transient frequency, testing its reported 27% reduction during ischemia-reperfusion.
• Model the relationship between transient adenosine concentration per event and the overall event frequency across experimental conditions.

Strengths

• Data collection spans a defined 4-hour experimental timeline with specific phases: 2h normoxia, 30min ischemia, 90min reperfusion.
• Quantitative results are provided, such as a 52% increase in total adenosine transients during ischemia-reperfusion compared to normoxia.
• Includes experimental data on pharmacological intervention with the A2A antagonist SCH442416, showing specific percentage reductions.

Limitations

• Sample size and number of experimental subjects (rats) are not specified, limiting assessment of statistical power.
• The dataset is derived from a single, controlled animal model study, which may limit generalizability to other species or conditions.
• Raw data format, column structure, and measurement granularity (e.g., time-series resolution) are unknown.

Provenance

Source

Dryad digital repository, authored by Mallikarjunarao Ganesana.

Collection Method

Electrochemical fast-scan cyclic voltammetry (FSCV) measurements in anesthetized rats.

Time Range

Experimental data collected over a continuous 4-hour period per subject.

Freshness

Data last updated in June 2020.

Geography

Laboratory study; geographic location not specified.