Zooplankton DNA Metabarcoding Data Comparing Three Genetic Markers

Featuring DNA metabarcoding results from a study comparing the performance of three genetic markers (nuclear 18S rDNA, mitochondrial COI, and mitochondrial 16S rDNA) for characterizing zooplankton assemblages. The research assessed taxonomic coverage, resolution, and correspondence with morphology-based identification. The dataset supports reproducibility analysis, as COI amplicons sequenced on separate runs showed highly reproducible operational taxonomic units.

Use Cases

• Compare taxonomic coverage across zooplankton phyla using data from the mitochondrial COI and nuclear 18S markers.
• Assess species-level resolution by analyzing the threefold greater taxa resolved to species by mitochondrial COI compared to 18S.
• Examine patterns of beta-diversity revealed by all three markers to identify differing contributing taxa.
• Model the relationship between biomass and sequence reads for calanoid copepod families, comparing the strength of correlation across markers.
• Evaluate the reproducibility of operational taxonomic units representing >0.1% of reads per sample from COI amplicons sequenced on separate runs.

Strengths

• Data directly compares three established metabarcoding markers (18S, COI, 16S) for a standardized assessment.
• Includes analysis of reproducibility for COI operational taxonomic units representing >0.1% of reads.
• Study evaluates multiple performance dimensions: taxonomic coverage, resolution, beta-diversity patterns, and biomass-read relationships.

Limitations

• The specific sample size, number of rows, columns, and file formats are not provided in the input.
• Data is from a specific methodological study; its generalizability to other ecosystems or taxonomic groups is untested.
• The dataset's temporal and geographic coverage is not specified, limiting analyses of environmental change over time or space.

Provenance

Source

Dryad digital repository.

Collection Method

DNA metabarcoding of zooplankton assemblages using three genetic markers, with comparison to morphology-based identification.

Freshness

Last updated on 2020-06-24.