E. coli Peptidoglycan Cross-Linking and β-Lactam Resistance Factors

This microbiological study by Christiane Bouchier (2020) provides experimental data on the formation of 3→3 peptidoglycan cross-links in Escherichia coli. The records document the interaction between YcbB production and (p)ppGpp alarmone synthesis that facilitates bypass of standard D,D-transpeptidase activity. It includes observations on the enzymatic roles of RelA, PBP1b, and DacA in conferring β-lactam resistance.

Use Cases

• Analyzing the correlation between YcbB production levels and β-lactam resistance phenotypes
• Evaluating the role of (p)ppGpp alarmone synthesis by RelA in antibiotic tolerance
• Investigating the glycosyltransferase activity of PBP1b in the context of 3→3 cross-link formation

Strengths

• CC0-1.0 public domain license for unrestricted reuse
• Documents the specific YcbB-mediated bypass mechanism for D,D-transpeptidase activity
• Includes experimental observations for four distinct enzyme activities (YcbB, RelA, PBP1b, DacA)

Limitations

• Unknown file formats and data structure
• Strain-specific focus limited to Escherichia coli
• Lack of explicit column headers in the source metadata

Provenance

Source

Dryad Digital Repository

Collection Method

Laboratory experiment

Freshness

Last updated in June 2020; no subsequent updates recorded.