Metabolic Subpopulations: Mass Spectrometry of 12C and 13C Labeled Cells

Mass spectrometry measurements of amino acid composition in cells grown in 12C- and 13C-labeled sugars, published by Christine M. DeGennaro in 2020. The data enables the quantification of metabolic variability and sugar usage within cell populations to identify subpopulations.

Use Cases

• Inferring metabolic heterogeneity using 12C/13C isotope ratios
• Quantifying sugar usage variability across cell populations
• Validating mass spectrometry analysis methodologies for subpopulation identification

Strengths

• Uses dual 12C and 13C labeling to track sugar usage
• Provides a method for inferring single-cell heterogeneity from population-level data
• CC0-1.0 public domain license

Limitations

• Unknown record count and column structure
• Specific to amino acid composition and sugar metabolism
• Temporal staleness as data was last updated in 2020

Provenance

Source

Christine M. DeGennaro

Collection Method

Mass spectrometry of cells grown in labeled sugar mixtures

Freshness

Last updated June 2020; no scheduled updates.