Dengue Virus Infection Dynamics in Mouse Dermis Immune Cells

This dataset originates from a study investigating early Dengue virus (DENV) infection in the dermis of interferon-α/β receptor-deficient (Ifnar–/–) mice. It captures immune cell dynamics, including initial infection of resident dendritic cells and macrophages, followed by infection of recruited monocytes and monocyte-derived dendritic cells. The study identifies the dermis as the main site of early DENV replication.

Use Cases

• Analyze the temporal infection patterns of CD103+ classical dendritic cells, Ly6C– CD11b+ cDCs, and macrophages in the dermis 12-24 hours post-inoculation.
• Model the recruitment and differentiation of Ly6Chigh monocytes expressing CCR2 into Ly6C+ CD11b+ monocyte-derived DCs (moDCs) that become major virus targets after 48 hours.
• Compare DENV infection levels between hematopoietic cells in the dermis and Langerhans cells in the epidermis, which showed a ~100-fold difference.
• Investigate the two-wave infection strategy of DENV, first targeting resident immune cells and then exploiting recruited monocytes to increase infection targets.

Strengths

• Data is derived from a controlled experimental model using Ifnar–/– mice, which have normal skin cell populations and are susceptible to intradermal DENV infection.
• The study provides a detailed temporal resolution of infection, tracking immune cell targets at specific intervals (12-24 hours and 48 hours post-inoculation).
• Findings are based on comparative analysis, such as the ~100-fold greater infection in dermal hematopoietic cells versus epidermal Langerhans cells.

Limitations

• Data is generated from a specific mouse model (Ifnar–/–), which may not fully recapitulate human immune responses to DENV infection.
• The dataset scope is limited to early infection dynamics in the skin and does not cover systemic infection or later disease stages.
• Sample sizes and specific quantitative data points (e.g., exact cell counts, replication rates) are not provided in the available description.

Provenance

Source

Dryad

Collection Method

Experimental data from intradermal DENV infection in Ifnar–/– mice, using adoptive cell transfer and flow cytometry analysis.

Time Range

Covers early infection time points post-inoculation (12-24 hours and 48 hours).

Freshness

2020-06-24

Geography

Laboratory study; spatial coverage is the mouse dermis.