Fluorescence Data for Fe3+ and Ascorbic Acid Sensing in Biological Samples

Experimental data from a study on nitrogen and chlorine co-doped carbon dots (Orn-CDs) used as a fluorescent nanoprobe. The dataset supports quantitative assays for Fe3+ ions with a detection limit of 95.6 nmol L-1 and for ascorbic acid with a limit of 137 nmol L-1. The method was validated for sensing in human serum and urine specimens and for imaging Fe3+ in living cells.

Use Cases

• Train a regression model to predict Fe3+ concentration from Orn-CDs fluorescence intensity data within the 0.3-50 µmol L-1 range.
• Analyze the fluorescence recovery signal of Orn-CDs to quantify ascorbic acid concentration in the 0.5-10 µmol L-1 range.
• Validate the nanoprobe's performance by comparing sensing results from human serum and urine specimen data.
• Assess the photostability and cytotoxicity metrics of the Orn-CDs for evaluating their suitability in cell imaging applications.

Strengths

• The nanoprobe achieved a low detection limit of 95.6 nmol L-1 for Fe3+ ions.
• The Orn-CDs exhibited a high fluorescence quantum yield of 60%.
• Experimental validation was performed in complex biological matrices including human serum and living cells.

Limitations

• The dataset scope is limited to the specific Orn-CDs nanoprobe and its interaction with Fe3+ and ascorbic acid.
• Sample size and the number of experimental replicates are not specified in the provided description.
• The data is from a single study published in 2020 and may not reflect more recent methodological advances.

Provenance

Source

Dryad digital repository, authored by Jin Li.

Collection Method

Data generated from a one-step hydrothermal synthesis of carbon dots and subsequent fluorescence spectroscopy and imaging experiments.

Freshness

Last updated 2020-06-24.